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Objective :To study influence of exercise rehabilitation on serum levels of N terminal pro brain natriuretic peptide (NT‐proBNP) and high sensitive cardiac troponin T (hscTnT) and cardiac function indexes in patients with chronic heart failure (CHF).Methods :A total of 140 CHF patients treated in our hospital were randomly and equal‐ly divided into routine group (received routine treatment ) and exercise rehabilitation group (received exercise reha‐bilitation based on routine group ) ,both groups were treated for three weeks .Serum levels of NT‐proBNP ,hscTnT and cardiac function indexes were measured and compared between two groups before and after rehabilitative inter‐vention .Results :Compared with routine group after three‐week treatment ,there were significant reductions in ser‐um levels of NT‐proBNP [ (2. 60 ± 0. 53) μg／L vs.(2. 11 ± 0.52) μg／L] ,hscTnT [ (0.38 ± 0. 12) μg／L vs.(0. 30 ± 0.09) μg／L] ,left ventricular end‐diastolic dimension (LVEDd) [ (56.78 ± 6.21) mm vs.(50. 10 ± 4.21) mm] , left ventricular end‐systolic dimension (LVESd) [ (48. 11 ± 5. 88) mm vs.(35.00 ± 6.20) mm] ,left atrial diameter (LAD) [ (44. 01 ± 5.69) mm vs.(34.26 ± 6.09) mm] and left ventricular mass index (LVMI) [ (185.01 ± 31. 34) g／m2 vs.(125.89 ± 43. 52) g／m2 ] ,and significant rise in LVEF [ (43.10 ± 6.21)% vs.(52.13 ± 5.10)%] and left ventricular early／late diastolic peak flow velocity (E／A) [ (0. 94 ± 0. 44) vs.(1.40 ± 0.56)] in exercise rehabilita‐tion group , P＝0.001 all .Conclusion :Exercise rehabilitation can significantly reduce serum NT‐proBNP and hscTnT levels ,improve cardiac function in CHF patients .
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Objective :To explore influence of comprehensive rehabilitation intervention on serum NT-proBNP level , anxiety and depression in AMI patients .Methods : A total of 90 AMI patients treated in our hospital were selected , randomly and equally divided into routine rehabilitation (RR) group and comprehensive rehabilitation (CR) group , both groups were intervened for six months .Serum NT-proBNP level within 6h after onset ,2d ,6d ,12d and one month after intervention ,scores of Hamilton rating scale for anxiety (HAMA) ,Hamilton rating scale for depres-sion (HAMD) and general quality of life inventory (GQOLI )-74 before ,and one ,three , six months after inter-vention were measured and compared between two groups .Results : Compared with RR group ,there was significant reduction in serum NT-proBNP level [6d : (3126-33 ± 91-32 ) ng／L vs.(1799-16 ± 76-83 ) ng／L , one month :(1358-91 ± 53-01) ng／L vs.(505-13 ± 38-93) ng／L] on 2d ,6d ,12d and one month after intervention , P＝0-001 all ;significant reductions in scores of HAMA and HAMD [six months :HAMA ,(19-8 ± 1-6) scores vs.(10-3 ± 1-9) scores ;HAMD ,(20-2 ± 1-9) scores vs .(11-6 ± 1-5) scores] on three and six months after intervention , P＝0-001 all ;significant rise in scores of physical function [ (48-4 ± 1-8) scores vs .(49-7 ± 1-2) scores] and material life [ (51-5 ± 1-1) scores vs .(54-4 ± 1-9) scores] on three months after intervention ,and all dimension scores of GQOLI-74 on six months after intervention in RR group , P＝0-001 all.Conclusion : Comprehensive rehabilitation intervention can significantly reduce serum NT-proBNP level , improve anxiety and depression in AMI patients , which contributes to improving quality of life in these patients .It′s worth extending .
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OBJECTIVE@#To investigate the BRAF gene mutations in ameloblastic fibroma (AF), and to further analyze the relationship between the BRAF mutation and clinical characteristics so as to provide new reference to the study of AF's molecular pathology.@*METHODS@#Sixteen cases diagnosed as AF at the Department of Oral Pathology, Peking University School of Stomatology between January 1990 and December 2017 were collected. Genomic DNA was extracted from formalin-fixed, paraffin embedded tissues using the QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. Polymerase chain reaction (PCR) and direct sequencings were used to detect the BRAF gene mutations. The clinicopathological data, such as the age, location of the lesion, symptoms and treatments were retrospectively analyzed.@*RESULTS@#The sixteen cases of AF involved nine women and seven men aged 2-67 years. Three lesions occurred in the maxilla and thirteen in the mandible. The most common presenting symptom of AF was a painless slowly enlarging mass with swelling. Ten patients received conservative treatment and the other six patients received radical surgery. Three cases relapsed during the study period. BRAF gene mutation was found in sixteen of all the sixteen samples analyzed (100%). The BRAF mutation was a point mutation with a thymine-adenine transversion at nucleotide 1 799 of 15 exons, resulting in a change at residue 600 that substituted glutamine for valine. This mutation was the strongest activator of the downstream RAS/RAF/MEK/ERK-MAPK signaling pathway. This helped to bring about a gain-of-function mutation due to a V600E substitution. Many studies identified that BRAF regulated survival, apoptosis, and proliferation of cells by inducing MAPK pathways activation. For the existing cases, none of the age, sex, location, recurrence and treatments had a statistically significant correlation with BRAF mutation.@*CONCLUSION@#Our findings demonstrated high prevalence of BRAF V600E mutation in AF. The pathogenic role remains to be clarified．.
Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Exons , Fibroma/genetics , Mutation , Proto-Oncogene Proteins B-raf/genetics , Retrospective StudiesABSTRACT
Objective: To explore the clinical effect of the treatment of menstrual migraine (MM) by acupuncture taichong and yongquan points. Methods: We selected 90 patients with confirmed diagnosis of MM from January 2017 to January 2018 and randomly divided them into three groups: acupuncture treatment group, drug therapy, and acupuncture + medication group, with 30 cases in each group. In the acupuncture treatment group, the patients were treated by using acupuncture taichong and yongquan points. Those in the drug treatment group received oral medication of fluoroguirizine hydrochloride capsules. The combined therapy group received both body acupuncture treatment and oral fluoroguirizine hydrochloride capsule treatment. We compared the total headache score, the migraine specific quality of life questionnaire (MSQ), plasma calcitonin gene-associated peptide (CGRP) and 5-hydroxytryptamine (5-HT) before and after treatment. The clinical efficacy of the three groups was compared. Results: Before treatment, the headache score, MSQ, CGRP and 5-HT in the three groups did not differ (P>0.05). After treatment, the headache score, MSQ, CGRP and 5-HT of the three groups had decreased (P<0.05). Compared with the acupuncture treatment group and the drug therapy, the headache score, MSQ, CGRP and 5-HT of the three groups decreased in the acupuncture + drug therapy group (P<0.05). The total effective rate of the combination group was significantly higher than that of the other two groups (P<0.05). Conclusion: The clinical effect of acupuncture taichong and yongquan points on the treatment of menstrual migraine is significant. It can significantly improve the patients' headache symptoms and lift their quality of life.
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<p><b>OBJECTIVE</b>To investigate the comparison method on internal control of hematology analyzer by using fresh blood.</p><p><b>METHODS</b>The hematology analyzer with well function was selected as the reference analyzer, fresh blood samples from healthy subjects were measured by reference analyzer and the values were used to calibrate compared hematology analyzers. The acceptable limits of relative deviation of WBC,RBC, HGB,HCT, PLT were established by comparative experiments during three months. The results of fresh blood samples from patients with low/medium/high levels measured by compared analyzer were compared with those from reference analyzer, the relative deviation of WBC, RBC, HGB, HCT, PLT was calculated respectively. The internal quality control charts in laboratory information system were established, with date as x-axis, relative deviation as y-axis. The acceptable relative deviation limits were set to be +/-2 s, and to be used for laboratory quality control.</p><p><b>RESULT</b>The relative deviation of WBC, RBC, HGB, HCT, PLT with high, medium, low levels were(0.75+/-2.964)%, (1.19+/-2.488)%,(1.43+/-2.439)%; (-0.39+/-1.327)%, (-0.26+/-1.297)%, (-0.35+/-1.095)%û(-0.43+/-1.393)%, (-0.17+/-1.139)%, (0.24+/-1.166)%û(-.43+/-1.362)%, (-0.36+/-1.381)%, (-0.57+/-1.299)%û(-0.93+/-4.330)%,(0.04+/-4.118)%, (-0.41+/-4.149)%, respectively in 2006. As the second instrument, the compared analyzer was involved in College of American Pathologists Proficiency Testing with satisfactory results, the bias of WBC,RBC, HGB, HCT, PLT were within (-0.5 approximately 5.1)%, (-1.0 approximately 1.6)%, (-1.7 approximately 1.4)%, (-1.5 approximately 1.3)%, (-4.5 approximately 7.4)%, respectively.</p><p><b>CONCLUSION</b>The quality control on compared hematology analyzer can be effectively, conveniently and economically performed using this method.</p>
Subject(s)
Humans , Autoanalysis , Methods , Blood , Erythrocyte Count , Hematology , Methods , Reference Standards , Hemoglobins , Leukocyte Count , Platelet Count , Quality Control , Reference Standards , Weights and MeasuresABSTRACT
<p><b>OBJECTIVE</b>To investigate the prevalence of aminoglycoside resistance and genotyping of acetyltransferase in Escherichia coli.</p><p><b>METHODS</b>Resistance phenotypes to 12 antibiotics of 44 Escherichia coli isolates were analyzed using agar dilution method and 3 aminoglycoside resistance genes aac(3)-I, II and aac(6')-I were determined by PCR method.</p><p><b>RESULTS</b>In 44 clinical isolates, the occurrence of ESBLs was 45.45%, resistance rates were discrepant for amikacin (18.18%), gentamicin (56.82%) and tobramycin (61.36%), the prevalence of phenotype TG (tobramycin and gentamicin) indicative of aac(3)-II production and TGA (tobramycin, gentamicin and amikacin) indicative of aac(6')-I production were 36.36% and 18.18%, respectively. The most common aminoglycoside resistance genotype of acetyltransferase was aac(3)-II (52.27%) and aac(6')-I was lower (29.55%), but no aac(3)-I was detected.</p><p><b>CONCLUSION</b>At least 2 acetyltransferase genes exist in this area i.e. aac(3)-II and aac(6')-I.</p>